File Name: genetic analysis and mapping in bacteria and bacteriophages .zip
- Horizontal Gene Transfer
- Genetic Analysis of Bacteriophages
- Genetic variation in prokaryotes
- Bacterial conjugation
Horizontal Gene Transfer
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WordPress Shortcode. Published in: Science. Full Name Comment goes here. Are you sure you want to Yes No. Nilakshi Bhattacharjee. Sayed Sajid Ali Kazmi. Vishal Patel. Ramesh Patel. Show More.
No Downloads. Views Total views. Actions Shares. No notes for slide. Genetic Analysis and Mapping in Bacteria and Bacteriophages 1. Warren and Marshall Nobel Prize 5. Methods for bacterial growth 7. Replica Plating Transfer required physical contact McGraw Hill Mechanisms of bacterial gene transfer Mechanism of transfer 1. Relaxosome is produced 2.
Relaxosome recognizes the origin of transfer 3. T DNA is separated, but bound to relaxase protein 2. Complex called nucleoprotein 3. Complex recognized by coupling factor, fed through exporter F factor transfer 1. Relaxase joins ends to produce circular molecule 2. Single strands of F factor are in both cells DNA replication Hfr mediated conjugation Pro: proline Lac: lactose Mapping via Interrupted Mating Mapping of the E.
Permission required for reproduction or display What is going on with U-tube? Permission required for reproduction or display Contains the genetic material Used for attachment to the bacterial surface Plaque formation. You just clipped your first slide! Clipping is a handy way to collect important slides you want to go back to later. Now customize the name of a clipboard to store your clips. Visibility Others can see my Clipboard. Cancel Save.
Genetic Analysis of Bacteriophages
Skip to content. Skip to navigation. This is called vertical gene transfer and is why you have half of the charecteristics of your mother, and half of your father. Bacteria and some lower eukaryotes are unique in that they can pass DNA from one cell of the same generation to another. We refer to this as Horizontal Gene Transfer see diagram below. Conjugation is the transfer of DNA directly from one cell to another through cell-cell contact.
Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells. It is a mechanism of horizontal gene transfer as are transformation and transduction although these two other mechanisms do not involve cell-to-cell contact. Classical E. However, it is not sexual reproduction, since no exchange of gamete occurs, and indeed no generation of a new organism : instead an existing organism is transformed. During classical E. The genetic information transferred is often beneficial to the recipient. Benefits may include antibiotic resistance , xenobiotic tolerance or the ability to use new metabolites.
Essentials of Genetics - Chapter 8 - Genetic Analysis and Mapping in Bacteria and Bacteriophages.
Genetic variation in prokaryotes
Metrics details. Aquaculture is the fastest growing sector of food production worldwide. However, one of the major reasons limiting its effectiveness are infectious diseases among aquatic organisms resulting in vast economic losses. Fighting such infections with chemotherapy is normally used as a rapid and effective treatment.
Genetics of Microbes pp Cite as. The study of bacteriophages has had a crucial influence on many areas of genetics and molecular biology. Phages show great variation in structure, and examples of some of these are shown in Table 4.
NCBI Bookshelf. Modern Genetic Analysis.
Bacteria of the genus Pseudomonas are genetically diverse and ubiquitous in the environment. Like other bacteria, those of the genus Pseudomonas are susceptible to bacteriophages which can significantly affect their host in many ways, ranging from cell lysis to major changes in morphology and virulence. Insights into phage genomes, evolution, and functional relationships with their hosts have the potential to contribute to a broader understanding of Pseudomonas biology, and the development of novel phage therapy strategies. Here we provide a broad-based comparative and evolutionary analysis of complete Pseudomonas phage genome sequences available in online databases.
NCBI Bookshelf. Modern Genetic Analysis. New York: W. Freeman; Conjugation is merely the fusion of two compatible bacterial cells.
Gene mapping describes the methods used to identify the locus of a gene and the distances between genes. The essence of all genome mapping is to place a collection of molecular markers onto their respective positions on the genome. Molecular markers come in all forms. Genes can be viewed as one special type of genetic markers in the construction of genome maps, and mapped the same way as any other markers. There are two distinctive types of "Maps" used in the field of genome mapping: genetic maps and physical maps.
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